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Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
Engineering doi: 10.1016/j.eng.2023.02.016
Keywords: Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary gel
Removable Dyes—The Missing Link for In-Depth N-Glycan Analysis via Multi-Method Approaches Article
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
Engineering 2023, Volume 26, Issue 7, Pages 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016
As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans' immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.
Keywords: Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary
Jian LU,Paul S. WILLS,P. CHRIS WILSON
Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 3, Pages 477-481 doi: 10.1007/s11783-015-0820-z
Keywords: off-flavor/odor compounds liquid-liquid microextraction gas chromatography-tandem mass spectrometry
Liang ZHU, Jingkang WANG
Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 3, Pages 322-328 doi: 10.1007/s11705-013-1348-z
Keywords: tobramycin aminoglycoside refractive index detector high-performance liquid chromatography ion-pairing
WEI Yimei,ZHANG Yuan,XU Jian,GUO Changsheng,LI Lei,FAN Wenhong
Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 3, Pages 357-371 doi: 10.1007/s11783-013-0580-6
Keywords: antibiotics liquid chromatography–tandem mass spectrometry (LC-MS/MS) water sediment fish muscle
Feng Zhu, Zhijian Yao, Wenliang Ji, Deye Liu, Hao Zhang, Aimin Li, Zongli Huo, Qing Zhou
Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 3, doi: 10.1007/s11783-020-1228-y
Keywords: Hydrophilic resin Solid phase extraction Pharmaceuticals and personal care product Ultra-performanceliquid chromatography and tandem mass spectrometry Environmental water
Shengguo DENG, Zeyuan DENG, Yawei FAN, Jing LI, Rong LIU, Dongmei XIONG,
Frontiers of Chemical Science and Engineering 2009, Volume 3, Issue 4, Pages 375-382 doi: 10.1007/s11705-009-0247-9
Keywords: two-phase-solvent flavonoid glycoside high-speed counter-current macroporous counter-current chromatography
Chengkun WANG, Xiaojian ZHANG, Jun WANG, Chao CHEN
Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 6, Pages 770-777 doi: 10.1007/s11783-012-0412-0
Keywords:
Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 5, doi: 10.1007/s11783-020-1338-6
• N-Cl-DCAM, an emerging N-DBP in drinking water was investigated.
Keywords: N-chloro-2 2-dichloroacetamide Liquid chromatography with tandem mass spectrometry Precursors Removal
Yue-E SUN, Wenshui XIA, Xueyan TANG, Zhiyong HE, Jie CHEN,
Frontiers of Chemical Science and Engineering 2009, Volume 3, Issue 4, Pages 407-412 doi: 10.1007/s11705-009-0255-9
Keywords: high-performance liquid tension different spectrometry chromatography
Hydrophilic/underwater superoleophobic graphene oxide membrane intercalated by TiO
Zhichao Wu, Chang Zhang, Kaiming Peng, Qiaoying Wang, Zhiwei Wang
Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 3, doi: 10.1007/s11783-018-1042-y
Keywords: Hydrophilic Superoleophobic Graphene oxide Membrane Titanium dioxide nanotubes Oil-water separation
Yong QIU, He JING, Hanchang SHI,
Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 3, Pages 301-310 doi: 10.1007/s11783-010-0236-8
Keywords: perfluorocarboxylic acids (PFCAs) perfluoroalkyl sulfonates (PFASs) Lake Taihu area ultra performance liquidchromatography with tandem mass spectrometry (UPLC-MS)
Heng Fang, Aihua Zhang, Xiaohang Zhou, Jingbo Yu, Qi Song, Xijun Wang
Frontiers of Medicine 2020, Volume 14, Issue 5, Pages 651-663 doi: 10.1007/s11684-019-0709-5
Keywords: metabolomics liquid chromatography-mass spectrometry metabolites metabolic pathways
Eniko Haaz, Botond Szilagyi, Daniel Fozer, Andras Jozsef Toth
Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 5, Pages 913-927 doi: 10.1007/s11705-019-1877-1
Keywords: hydrophilic pervaporation non-ideal mixture modelling extractive heterogeneous-azeotropic distillation
Experimental investigations of frost release by hydrophilic surfaces
Zhongliang LIU, Lingyan HUANG, Yujun GOU, Yaomin LIU
Frontiers in Energy 2010, Volume 4, Issue 4, Pages 475-487 doi: 10.1007/s11708-010-0114-x
Keywords: frost formation hydrophilicity heat and mass transfer performance
Title Author Date Type Operation
Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
Journal Article
Removable Dyes—The Missing Link for In-Depth N-Glycan Analysis via Multi-Method Approaches
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
Journal Article
Trace analysis of off-flavor/odor compounds in water using liquid-liquid microextraction coupled withgas chromatography—positive chemical ionization-tandem mass spectrometry
Jian LU,Paul S. WILLS,P. CHRIS WILSON
Journal Article
Fast determination of tobramycin by reversed-phase ion-pair high performance liquid chromatography with
Liang ZHU, Jingkang WANG
Journal Article
Simultaneous quantification of several classes of antibiotics in water, sediments, and fish muscles by liquidchromatography–tandem mass spectrometry
WEI Yimei,ZHANG Yuan,XU Jian,GUO Changsheng,LI Lei,FAN Wenhong
Journal Article
An efficient resin for solid-phase extraction and determination by UPLCMS/MS of 44 pharmaceutical personal care products in environmental waters
Feng Zhu, Zhijian Yao, Wenliang Ji, Deye Liu, Hao Zhang, Aimin Li, Zongli Huo, Qing Zhou
Journal Article
Application of high-speed counter-current chromatography coupled with high performance liquid chromatography
Shengguo DENG, Zeyuan DENG, Yawei FAN, Jing LI, Rong LIU, Dongmei XIONG,
Journal Article
Characteristics and removal mechanism of the precursors of N-chloro-2,2-dichloroacetamide in a drinking water treatment process at Taihu Lake
Journal Article
Effects of fatty acid chain length and degree of unsaturation on the surface activities of monoacyl trehaloses
Yue-E SUN, Wenshui XIA, Xueyan TANG, Zhiyong HE, Jie CHEN,
Journal Article
Hydrophilic/underwater superoleophobic graphene oxide membrane intercalated by TiO
Zhichao Wu, Chang Zhang, Kaiming Peng, Qiaoying Wang, Zhiwei Wang
Journal Article
Perfluorocarboxylic acids (PFCAs) and perfluoroalkyl sulfonates (PFASs) in surface and tap water around Lake Taihu in China
Yong QIU, He JING, Hanchang SHI,
Journal Article
High-throughput metabolomics reveals the perturbed metabolic pathways and biomarkers of Yang Huang syndrome as potential targets for evaluating the therapeutic effects and mechanism of geniposide
Heng Fang, Aihua Zhang, Xiaohang Zhou, Jingbo Yu, Qi Song, Xijun Wang
Journal Article
Combining extractive heterogeneous-azeotropic distillation and hydrophilic pervaporation for enhanced
Eniko Haaz, Botond Szilagyi, Daniel Fozer, Andras Jozsef Toth
Journal Article